In terms of reproductive and other adverse outcomes after exposure to polychlorinated biphenyls (PCBs), dioxins, and furans, the mink (Mustela vison) is one of the most sensitive mammals. Our objective was to determine if there are differences in the concentrations of total mercury (Hg), total PCBs, and dioxin-furan toxic equivalents (TEQs) between mink living in and out of the Rochester Embayment of Lake Ontario (RELO) Area of Concern (AOC) and between mink living near the shore of Lake Ontario and inland. Concentrations of total Hg in the brain, total PCBs and dioxin-furan TEQs in adipose, and total PCBs in liver were significantly higher for mink living near the shore of Lake Ontario than inland. For mink living in and out of the AOC, differences in total PCBs and dioxin-furan TEQs in adipose and the liver were substantial but not significant. Correlations among concentrations of total Hg, total PCBs, and dioxin-furan TEQs in mink were high. Our results suggest that contamination of mink living near the southern shore of Lake Ontario primarily comes from contact with the Lake Ontario food web, not from sources in the RELO AOC. © 2009 Springer Science+Business Media, LLC.